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Light Sheet Microscope

源自EMBL實(shí)驗(yàn)室的原創(chuàng)Lightsheet技術(shù)

MuVi-SPIM

IMAGING LIFE IN THE FAST LANE

Latest sCMOS camera technology and optimized combinations of objective lenses enable you to obtain the best from your sample: data you always wanted but could never get.

  • Intrinsic 3D optical sectioning

  • Large field of view

  • Close-to-confocal resolution

  • Very high imaging speed

  • Highest sensitivity and minimal noise

360° ILLUMINATION AND DETECTIONThe four objective lenses allow four combinations of illumination and detection. This provides four orthogonal views of the specimen without the need for sample rotation, enabling:

  • Multiple view imaging of large samples at unprecedented speed

  • Long-term stability

  • Time series over several days

  • Robust image registration

  • Efficient data fusion and visualization

Laser
  • Laser combiner with six laser positions

  • Available laser lines 445, 488, 515, 532, 561, 594, 642, and 685 nm with 50 mW each

  • Fast modulation and high extinction

Illumination optics
  • Two identical illumination arms

  • Chromatic correction from 440 to 660 nm

  • Light-sheet generation by beam scanning

  • Variable light-sheet thickness (optional)

  • Water-dipping objective lenses (see below)

Detection optics
  • Two identical detection arms

  • Water-dipping objective lenses (see below)

  • Fast filter wheels with 10 positions and 50 ms between adjacent positions

  • Filters adapted to the selected laser lines

  • Variable magnification (optional)

  • High-speed sCMOS cameras Hamamatsu Orca Flash 4.0 with 2048 x 2048 pixels of 6.5 μm x 6.5 μm size

Objective lens mounting unit
  • High precision mounting unit

  • Fast exchange of the unit with high precision

  • Mounting chamber with temperature control (see below)

  • 2 x Nikon CFI Plan Fluor 10XW 0.3 NA water immersion for illumination

  • 2 x Nikon CFI Apo LWD 25XW 1.1 NA water immersion for detection

  • Other objective lenses on request

Sample chamber and stage
  • Water-sealed inert PEEK plastic chamber, autoclavable and biocompatible

  • Sample supported from below for improved stability

  • Easy access from above for sample mounting, injections, etc.

  • Fast and precise temperature control, range 15-40°C

  • High-precision XYZ and fast rotation stage

  • XYZ piezo crawler stage with 100 nm resolution and 8.5 mm (X) and 1.2 mm (YZ) travel range for fast sample positioning and stack acquisition

  • Custom-made rotation stage with > 360 degrees/second speed

Electronics, Computer, Software
  • All electronic components included in the microscope housing

  • Embedded microscope software with open communication interface

  • Open GUI control for interactive control and microscope automation

  • Computer with 64 GB RAM, Intel Dual Six Core CPU

  • High-speed RAID controller for data streaming

  • 8 x 4 TB local storage in RAID 0

  • One 40 inch monitor

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